Published by:
CGP EDU Academic Team
Published on: August 21, 2026
Select the correct option which shows the most appropriate temperatures of three different steps of PCR Mechanism
Denaturation | Primer annealing | Primer extension | |
(a) | |||
(b) | |||
(c) | |||
(d) |
Text Solution
Verified by ExpertsThe correct answer is:
C
Denaturation: This step needs a high heat of 94–96°C. This high heat splits the DNA strands apart.
Primer annealing: This step needs a lower heat of 40–60°C. This cool temperature lets primers stick to the DNA.
Primer extension: This step needs a medium heat of 72°C. This temperature helps the enzyme copy the DNA quickly.
Prepare Smarter with CGP Edu
Get practice questions, solutions, and test series in one place.
Write a Review
Share your experience with this question and solution.
Commentary
Send your comment, doubt, correction, or feedback to admin.
Similar Questions
Explore conceptually related problems
The energy required to ionize a hydrogen like ion in its ground state is 9 Rydberg's. What is the w…
A flask contains argon and oxygen in the ratio of 3:2 in mass and the mixture is kept at 27°C. The …
Hydrogen atom from excited state comes to the ground by emitting a photon of wavelength . The valu…
The activity of a radioactive material is 6.4 x 10 -4 curie. Its half life is 5 days. The activity …
The half life period of a radioactive substance is 60 days. The time taken for th of its original …
A radioactive sample decays times its original quantity in 15 minutes. The half-life of the sample…